Journal: The Journal of Neuroscience

Article Title: Effects of Early Visual Experience and Diurnal Rhythms on BDNF mRNA and Protein Levels in the Visual System, Hippocampus, and Cerebellum

doi: 10.1523/jneurosci.21-11-03923.2001

Figure Lengend Snippet: Figure 1. A, ECLIA measurements of BDNF protein concentrations in the SC, V1, retina, HIPP, and CBL of rats born and reared in a normal light cycle (NR; 14/10 hr light/dark cycle), constant darkness (DR), or constant light (LR). Similar measurements for SC and V1 are presented for hamsters at the right. All values are expressed as the percentage of normal. Error bars show SD. Numbers above each bar indicate the number of independent samples from which data were obtained (see Materials and Methods). B, RPA measurements of BDNF mRNA concentrations in rats in the same structures and under the same rearing conditions shown in A. All values are expressed as the percentage of normal. Numbers above each bar indicate the number of independent samples from which data were obtained (see Materials and Methods). Means and SEs of Phosphor- Imager pixel densities are indicated in Table 1. C, Scatter plot summa- rizing the relationship between changes in BDNF mRNA and BDNF protein induced in rats by DR ( filled symbols) and LR (open symbols). Asterisks in A and B indicate values in LR or DR animals that differ significantly from normal.

Article Snippet: One hundred fifty microliters of ECLIA reaction mixture (12.5 ng of biotinylated Rab-BDNF, 25 ng of Rab-BDNF-TAG, and 10 mg of Dynal 2.8 mm M-280 streptavidin magnetic beads diluted in Ca 21- and Mg 21-free Dulbecco’s PBS, pH 7.2, containing 3% BSA, 1.5% Tween 20, and 0.05% NaN3) were added to 100 ml of tissue supernatant unknowns aliquoted in 12 3 75 mm polypropylene tubes.

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